FIG 2.

Mutation of BRCT domain 4 attenuates the Brc1-Rhp18 interaction. (A) A schematic representation of published Brc1 point mutations (16) tested for physical interaction with the Mid domain of Rhp18 by yeast two-hybrid analysis. (B) Yeast two-hybrid results indicating that mutation of BRCT4 inhibits the interaction with the Mid domain of Rhp18. (C) Results from coimmunoprecipitation experiments demonstrating the interaction between full-length Brc1 and Rhp18, as well as the reduction in Rhp18 binding observed with TAP-Brc1-HYP307-9GFG versus TAP-Brc1.