FIG 3.

Efficient rescue of brc1Δ by expression of brc1-T672A but not brc1-HYP307-9GFG. (A) Functional evaluation of four brc1 alleles in response to MMS treatment in a brc1Δ genetic background suggests that brc1-HYP307-9GFG (16) retains more activity than brc1Δ but significantly less than the previously published γH2A binding mutant brc1-T672A (12), which rescued brc1Δ, as well as brc1⁺ and brc1-W298F,P301G. (B) Functional evaluation of the brc1 alleles in response to MMS treatment in the htaAQ brc1Δ genetic background, demonstrating the Brc1-Rhp18 interaction is more essential for Brc1 function in an overexpression situation than its ability to bind γH2A. In each panel, rows 1 and 3 contain cells transformed with empty pREP41X.