Skip to main content
. 2017 Oct 27;37(22):e00260-17. doi: 10.1128/MCB.00260-17

FIG 4.

FIG 4

Rhp18 and γH2A binding are required for efficient rescue of smc6-74 by Brc1 overexpression. (A) Results from smc6-74 suppression experiments comparing the rescue efficiency of the four brc1 alleles. The brc1-HYP307-9GFG allele that disrupts Brc1 binding to Rhp18 prevents Brc1 overexpression suppression of smc6-74. The brc1-T672A mutation that abrogates binding to γH2A impairs Brc1 overexpression suppression of smc6-74. (B) Results from smc6-74 htaAQ suppression experiments comparing the four brc1 mutations, supporting the MMS dose dependence for γH2A binding in mediating the scm6-74 rescue by Brc1. (C) Results from brc1 overexpression assays in the smc6-74 htaAQ rhp18Δ background, suggesting the failure of brc1-HYP307-9GFG to rescue smc6-74 is not completely explained by its inability to bind Rhp18. In each panel, rows 1 and 3 contain cells transformed with empty pREP41X.