FIG 3.

pUL31 expression in RK13 cell lines. RK13 cells stably expressing native pUL31 or the mutated proteins, as well as nontransgenic RK13 cells, were harvested by scraping them into the media. Cells were lysed in sample buffer, and proteins were separated in an SDS-10% polyacrylamide gel. After transfer to nitrocellulose, the blot was incubated with the monospecific anti-pUL31 rabbit serum and a monoclonal alpha-tubulin mouse antibody as loading control. Molecular masses of marker proteins are indicated on the left in kilodaltons.