FIG 3.

HSV-1 enters vestibular epithelia. (a) HE-stained section of epithelium from vestibular mucosa separated from the lamina propria by dispase II. (b) EM analysis of vestibular epithelia shows cell-cell contacts in the basal layer and a magnification of a desmosome. (c) Sections of infected vestibular epithelia (100 PFU/cell) stained for ICP0 (green) visualize viral spreading from basal to upper layers with DAPI (blue) as nuclear counterstain. (d) Sections of infected vestibular epithelia were costained for ICP0 (green) and CD1a (red) to visualize the distribution of Langerhans cells (LCs). No preferred infection of LCs was detected at 16 h p.i. The white line indicates the apical mucosal surface. Single immunofluorescence (c) and overlays (d) are shown. Bars represent 50 μm in panel a, 0.1 μm in panel b, and 50 μm in panels c and d.