FIG 4.

ZIKV infection does not alter the expression of tight junction and adherens junction proteins in HSeCs. (A and B) Total protein extracted from ZIKV-infected HSeCs (MOI of 1) was used to measure the levels of TJPs (ZO-1, occludin, and claudin) (A) and adherens junction proteins (β-catenin and JAM-A) and β-actin (B) by Western blotting using specific antibodies. The Western blot is representative of results from at least three independent infections. M, molecular marker. (C) Immunostaining of ZO-1 in mock- and ZIKV-infected HSeCs 48 h after infection shows comparable ZO-1 signals at the cell borders and cell-to-cell contact sites.