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. 2017 Oct 27;91(22):e01390-17. doi: 10.1128/JVI.01390-17

FIG 7.

FIG 7

Phosphorylation of E6AP at T485 confers its interaction with 14-3-3γ. (Left) PKA and 14-3-3 phosphoconsensus motifs. (Right) Direct interaction assay with purified 14-3-3γ. Purified GST fusion proteins were either untreated or subjected to phosphorylation (indicated as “P”) with PKA in the presence of nonradiolabeled ATP. They were then incubated with purified recombinant 14-3-3γ. (Upper) After extensive washing, the bound protein was detected by Western blotting using anti-14-3-3γ antibody. (Lower) Ponceau staining of the nitrocellulose membrane.