TABLE 1.
Summary of TC-μNS mutant propertiesa
| TC-μNS mutant | Location of CCPGCC tag | VFL formation | FlAsH-EDT2 labeled | Virus recovery | Protein colocalization compared to wild-type μNS |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| σNS | μ2 | λ1 | λ2 | σ2 | λ3 | |||||
| #1 | aa 28 | + | + | No | − | + | + | − | + | + |
| #2 | aa 132 | − | − | No | ND | ND | ND | ND | ND | ND |
| #4 | aa 334 | + | + | No | + | + | − | − | + | − |
| #5 | aa 491 | − | − | No | ND | ND | ND | ND | ND | ND |
| #6 | aa 614 | + | + | No | + | + | − | + | + | − |
| #7 | aa 545 | + | + | Yes | + | − | − | + | + | − |
| N-term | N terminus | + | + | No | − | + | + | + | + | + |
| C-term | C terminus | + | + | Yes | + | + | + | + | + | + |
a
−, no VFL formation or no FlAsH-EDT2 labeling or significantly diminished (P < 0.05) colocalization of TC-μNS and interacting proteins compared to wild-type μNS; +, VFL formation or FlAsH-EDT2 labeling or no significant decrease in colocalization of μNS and interacting proteins compared to wild-type μNS. ND, no data collected.