FIG 2.

IFI16, PML, and Sp100 expression in HCMV-Ab-treated macrophages. Medium alone and medium containing either an Ig pool, cell-free TB40/E, or the combination of cell-free TB40/E and an Ig pool were incubated at 37°C for 30 min. The amount of TB40/E was calculated to reach an MOI of 3. The four media were then added to macrophages (6 × 10⁵) in a volume of 600 μl for 1 h in 24-well plates. Cells were washed twice and were replenished with RMPI 1640 medium. At 24 h posttreatment, the total levels of IFI16 (A), PML, and Sp100 (B) were evaluated by Western blot analysis in cell lysates.