FIG 7.

HCMV-Ab-treated epithelial cells are not resistant to HCMV challenge. The four types of media described in the legend to Fig. 2 were added to ARPE-19 retinal pigment epithelial cells (1 × 10⁵) in a volume of 100 μl for 60 min (MOI, 3). Cells were washed twice and were incubated in their respective culture media. After 24 h, treated cells were either stained for quantitative evaluation of IEA positive cells (number in the top right corner of the images) or challenged by cell-free TB40/E in a volume of 100 μl for 60 min at an MOI of 3. Cells were then washed twice and were replenished with new medium. Twenty-four hours later, IEA expression was evaluated by indirect immunofluorescence.