Figure 1.

S. cerevisiae Enables Growth of Lactic Acid Bacteria

(A) Quantification of S. cerevisiae, L. lactis, and L. plantarum colony-forming units (CFUs) in monocultures and communities after 24 hr (pooled technical replicates of n = 3 biological replicates).

(B) Dynamics of L. plantarum and L. lactis growth in co-culture with S. cerevisiae (colored lines) and in monoculture (gray lines). Data shown as mean ± SD for three biological replicates.

(C) Growth of L. lactis (L.l.) and L. plantarum (L.p.) in close proximity and apart from an S. cerevisiae (S.c.) colony.

(D) Quantification of S. cerevisiae, L. lactis, and L. plantarum CFUs in monocultures and communities after 2 weeks of daily passaging (pooled technical replicates of n = 3 biological replicates). See also Figure S1.

(E) Effect of yeast-conditioned medium on LAB, normalized by yeast cell density. Barplot shows the mean and the dots represent pooled technical replicates from at least four independent experiments.