Figure 2.

Identification of Yeast Secretome Components that Enable Growth of LAB

(A) Conditioned medium assay design.

(B) Untargeted metabolomics workflow (flow injection analysis time of flight [FIA-TOF] mass spectrometry) as applied to the conditioned medium assay.

(C) Exo-metabolome dynamics of S. cerevisiae and L. plantarum revealed by untargeted metabolomics. Shown are the groups of ions with distinct profile shape. Cluster of metabolites potentially cross-fed from yeast to bacteria (bell-shaped curves) is highlighted in red. Data for one sample are shown, see also Figure S3 for summary statistics.

(D) Annotated metabolites produced by S. cerevisiae and consumed by LAB (with at least 2-fold change in both accumulation and decrease). See also Figure S3. Note that ion annotation based on accurate mass may be ambiguous; see Table S2 for complete annotation.