Figure 5.

FACS analysis of immune cells in the colonic lamina propria of KDG-knockout mice as compared to wild-type littermate controls. (a) Events are electronically gated on CD45⁺CD3⁺CD4⁺ cells, and cells within colored contour plots show staining for IFN-γ and IL-17A. (b) Box plots show percentages of CD4⁺ T cells producing IL-17, IL-17 and IFN-γ, or IFN-γ in the KDG-knockout mice. (c) Colonic lamina propria cells were isolated from the indicated knockout strains and wild-type controls and stained with anti-CD45, anti-CD11c, anti-CD11b, anti-CD103, anti-CD64, and anti–MHC II antibodies. Cells were electronically gated on CD45⁺CD11⁺MHC II⁺ cells and further subdivided by staining for CD103, CD11b, and CD64. Box plots show the percentages of CD103⁺ DCs, CD103⁺CD11b⁺ DCs, CD103⁻CD11b⁺ DCs, and CD64⁺ macrophages. Data shown are representative of four independent experiments. One-way ANOVA with Bonferroni's multiple comparison was performed. Box limits, first and third quartiles; line, median; whiskers, minimum and maximum values. Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001.