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(A) Comparison of PMN functional capacity following separation by C-sep and DTT techniques. NE release was significantly increased by PMA stimulation only for PMNs by C-sep. (B) Evaluation of NE increment by PMA stimulation separated by C-sep, DTT, and blood-borne neutrophils. NE release by airway PMNs isolated by C-sep was higher than NE release by airway PMNs isolated by DTT protocol but lower when compared to blood-borne neutrophils.
