Figure 2. Sucrose step gradients for tonoplast vesicle isolation.

Schematic representations of the sucrose step gradients prepared in step 5 for high purity, A, and high yield C protocols. Panels B and D show examples of the gradients after centrifugation in step 8. The interphases to be harvested are indicated with triangles. These gradients have been used to isolate membranes from mature red fruit.