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. Author manuscript; available in PMC: 2018 Mar 4.
Published in final edited form as: Nat Microbiol. 2017 Sep 4;2(11):1513–1522. doi: 10.1038/s41564-017-0019-0

Figure 4. CD81 negatively regulates cellular dNTP content through SAMHD1.

Figure 4

a) Jurkat T cells transfected with control or CD81 siRNA were infected with HIV-1 NL4-3 strain, and early RT products were measured by qPCR at the indicated times. Data are mean fold change ± SEM of 2 independent experiments performed in triplicate. In-box shows representative immunoblots of CD81 and tubulin as loading control, and the CD81/Tubulin signal ratio is indicated. b) Jurkat T cells pre-treated with 2μM of scramble or CD81pept for 5 days were infected with NL4-3 strain, and RT was analysed as in a. c) Mean fold change ± SEM of the dNTP content of Hela/R5 or Hela/R5 CRISPR/Cas9-CD81 cells (left graph, 4 independent experiments), and primary T lymphoblasts transfected with control or CD81 siRNA (right graph, 2 independent experiments) measured by a HIV RT-based dNTP assay. Analysis was performed by paired Student t-test, *** p<0.0001 (left) and *** p<0.0001 (right). d) dNTP content of Hela/R5 cells overexpressing GFP, CD81GFP or CD81∆cytGFP measured as in c. Data are mean fold change ± SEM from 3 independent experiments analysed by one-way ANOVA with Tukey’s post-test.