Table 1. SAGA is a conserved transcriptional co-activator complex organized into well-defined structural and functional modules.
Shown are the names of each subunit from Saccharomyces cerevisiae (sc), Saccharomyces pombe (sp), Drosophila melanogaster and Homo sapiens, according to the official nomenclature. Names in brackets are alternative, commonly used names. Paralogous subunits are separated with a “/” sign and are indicated if they have been described as part of SAGA. In most cases, paralogous subunits are mutually exclusive within SAGA complexes. Individual module subunits are coloured according to the code used in Figure 1. *The TBP binding module comprises both Spt3 and Spt8 in S. cerevisiae. In metazoans, no ortholog of scSpt8 has been found and the histone-fold domains of SUPT3H might rather contribute to SAGA structural core. #The bromodomain is found in S. cerevisiae Spt7, but not in its metazoan orthologs. @In S. pombe, there are two paralagous genes, tra1 and tra2, but only Tra1 is found in SAGA whereas Tra2 is specific for the NuA4 co-activator complex. Bromo: bromodomain; HFD: histone fold domain.
Functional tools | Orthologous SAGA complexes | Chromatin “reader” domains | Structural domains | |||
---|---|---|---|---|---|---|
S. cerevisiae | S. pombe | D. melanogaster | H. sapiens | |||
HAT module | Gcn5 | Gcn5 | KAT2 (GCN5) | KAT2A/KAT2B (GCN5/PCAF) | Bromo | |
Ada2 | Ada2 | Ada2b | TADA2b | SANT SWIRM | ||
Ngg1 (Ada3) | Ngg1 (Ada3) | Ada3 | TADA3 | |||
Sgf29 | Sgf29 | Sgf29 | SGF29 | Tudor (x2) | ||
DUB module | Ubp8 | Ubp8 | dNonstop | USP22 (UBP22) | ||
Sgf11 | Sgf11 | dSgf11 | ATXN7L3 | Nucleosome binding | ||
Sgf73 | Sgf73 | dATXN7 | ATXN7/ATXN7L1/L2 | |||
Sus1 | Sus1 | dE(y)2 | ENY2 | |||
Core structural module | Taf5 | Taf5 | WDA/TAF5L | TAF5L | WD40 | |
Taf6 | Taf6 | SAF6/TAF6L | TAF6L | HFD | ||
Taf9 | Taf9 | TAF9 | TAF9/TAF9b | HFD | ||
Taf10 | Taf10 | TAF10b | TAF10 | HFD | ||
Taf12 | Taf12 | TAF12 | TAF12 | HFD | ||
Spt7 | Spt7 | Spt7 | SUPT7L (STAF65G) | Bromo# | HFD | |
Hfi1 (Ada1) | Hfi1 (Ada1) | Ada1 | TADA1 | HFD | ||
Spt20 | Spt20 | Spt20 | SUPT20H | |||
TBP binding* | Spt3 | Spt3 | Spt3 | SUPT3H | HFD (x2) | |
Spt8 | Spt8 | - | - | WD40 | ||
TF binding module | Tra1 | Tra1@ | Nipped-A( Tra1) | TRRAP | ||
Splicing module | - | SF3B3 | SF3B3 | |||
- | SF3B5 | SF3B5 |