Skip to main content
. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Aug 25;1864(12):2369–2377. doi: 10.1016/j.bbamcr.2017.08.009

Figure 2. AMPK mediates the inhibitory effect of metformin on ALK2 signaling.

Figure 2

A. MEF cells, wildtype, LKB1 KO, or AMPKα1 & α2 KO, were treated with metformin (10mM) for 24 hours, followed by BMP6 (25ng/ml) for 30 min. B–C. FOP fibroblasts were infected with adenovirus in varying volumes for 48 hours. The virus expresses an active mutant of AMPKα1 (AMPK-CA) (B) or a dominant negative mutant of AMPKα1 (AMPK-DN) (C), both tagged with the flat epitope, or GFP as a control. The cells were treated with or without metformin (10mM) for 24 hours and followed with BMP6 (50ng/ml) for 30 min. Equal amounts of cell extracts (20μg) were blotted with antibodies, as indicated.