Figure 1.

Workflow in search for sQTLs. (A) The design of the recombinant inbred lines. Strains were initiated with eight founder strains A1–A8 with a diverse geographic origin. In the first generation, lines were intercrossed with each other and 10 F1 flies per genotype per sex were mixed together to establish the next generation. This mix went on until the 50th generation when flies were separated and Inbreeding continued for another 25 generations, leading to a total of ~1,600 completed recombinant inbred lines. After samples were treated w/o Pb, RNA-seq analysis was performed and two methods were used to target sQTLs: (B) the fraction of exon reads to transcript reads, and (C) Isoform dosage.