Figure 3.

LtaS is essential for the early stages of colony development. (A) Cells of PY79 (wt), GM21 (ΔltaS) and GM45 (ΔltaS, P_{hyper−spank}- ltaS) were grown on solid LB medium and incubated at 37°C for 20 h. Shown are typical colonies photographed using a binocular. Scale bar 0.5 mm. (B) The average diameter of colonies described in (A). At least 15 colonies were measured for each strain. Statistical significance was calculated using unpaired t-test. (C) Time lapse fluorescence images of wt AR16 (P_rrnE-gfp) (upper) and GM24 (ΔltaS, P_rrnE-gfp) (lower) developing colonies followed by CLSM at the indicated time points. Dashed circle at t = 300 min marks the radial area occupied by each developing colony. Scale bars 10 μm. ^***P < 0.001, Very significant.