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Palmitic acid (PA) dose-response curve at 24 h. Astrocyte cultures were treated with doses of 0.1, 0.25, and 0.5 mM of PA. Immunoblots were probed with antibodies toward glial fibrillary acidic protein (GFAP) in male (A), and female (B) astrocytes and interleukin 6 (IL-6) in male (C) and female (D) astrocytes. The average of three independent assays performed in duplicate is shown. Statistical significance: ∗∗p < 0.01.
