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. 2017 Oct 24;10:330. doi: 10.3389/fnmol.2017.00330

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Copyright © 2017 Frago, Canelles, Freire-Regatillo, Argente-Arizón, Barrios, Argente, Garcia-Segura and Chowen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of PA and 17β E2 on cell death and cell viability. Astrocyte cultures were treated with PA (0.5 mM), E2 (10^-10 M) or the combination of both. Cell death was assayed by ELISA in males (A) and females (B). Viability was assayed by crystal violet. The average of three independent assays performed in triplicate is shown. Statistical significance: ^∗p < 0.05 and ^∗∗p < 0.01.