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. 2017 Oct 24;8:1340. doi: 10.3389/fimmu.2017.01340

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Copyright © 2017 Embregts, Rigaudeau, Veselý, Pokorová, Lorenzen, Petit, Houel, Dauber, Schütze, Boudinot, Wiegertjes and Forlenza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression analysis of the local immune response after i.m. DNA vaccination against spring viremia of carp virus (SVCV). Carp were injected with 0.1 µg/g of either pcDNA3 or pcDNA3-SVCV-G. Muscle tissue at the site of injection was excised at 3 and 5 days postinjection (dpi). Expression of the indicated immune-relevant genes was normalized against the housekeeping gene s11 of the ribosomal subunit 40S and expressed relative to the unhandled control at time point 0 h. Asterisks (*) indicate significant differences (p < 0.05) between the pcDNA3 and pcDNA3-SVCV-G group at the respective time point as assessed by one-way ANOVA, followed by a Tukey post hoc test. Bars indicate average and SD of n = 3 fish per time point.