Table 1.
Primers used in real-time quantitative PCR.
| Primer | FW primer 5′–3′ | RV primer 3′–5′ | Acc. no. |
|---|---|---|---|
| Housekeeping genes | |||
| 40s | CCGTGGGTGACATCGTTACA | TCAGGACATTGAACCTCACTGTCT | AB012087 |
| β-actin | CAACAGGGAAAAGATGACACAGATC | GGGACAGCACAGCCTGGAT | CCACTBA |
| SVCV detection | |||
| svcv-n | TGAGGTGAGTGCTGAGGATG | CCATCAGCAAAGTCCCGGTAT | NC_002803 |
| Cytokines | |||
| cxca | CTGGGATTCCTGACCATTGGT | GTTGGCTCTCTGTTTCAATGCA | AJ421443 |
| cxcb1 | GGGCAGGTGTTTTTGTGTTGA | AAGAGCGACTTGCGGGTATG | AB082985 |
| cxcb2 | AGGCAGGTGCTTCTGTGCTGACA | TTCATGCATTTCCGCTCTGCGCT | JN104598 |
| il1β | AAGGAGGCCAGTGGCTCTGT | CCTGAAGAAGAGGAGGAGGCTGTCA | AJ245635 |
| il6a | CAGATAGCGGACGGAGGGGC | GCGGGTCTCTTCGTGTCTT | KC858890 |
| il6b | GGCGTATGAAGGAGTGAGGG | TGCTCCTCTCTCGGTCAGAT | KC858889 |
| tnfα | GCTGTCTGCTTCACGCTCAA | CCTTGGAAGTGACATTTGCTTTT | AJ311800 and AJ311801 |
| Transcription factors | |||
| stat1 | GAGACGGAGGAATCACC | GGATGTCTGGGTAAAGGTAG | KJ782028 |
| Interferons | |||
| ifnγ2a/2b | CGATCAAGGAAGATGACCCAGTC | GTTGCTTCTCTGTAGACACGCTTC | AM168523 |
| ifnφ1 | GCACGTATACAAAGATGAACC | TGATCCAAGGTCAAGACAAG | GQ168341 |
| ifnφ2 | TTGGTGTAAAAAAGGCAACC | GCTGCTTTCTCGTCATAATAC | JN741616 |
| Interferon-stimulated genes | |||
| mx1 | ACAATTTGCGGTCTTTGAGA | CCCTGCCATTTCTCTTCG | cypCar_00015892 |
| mx2 | GCTTACGGTCTCTGGGG | TGGTTTCATCTTTAGTTCTTATCATC | cypCar_00029512 |
| vip2 | CTGTCGGACACATCAGC | TCAATGGGCAAGACGAAA | cypCar_00024055 |
| pkr3 | CACGGTGTTTGAAAAGAGC | GACTGGGTCTCAGCATTC | cypCar_00039221 |
| isg15.2 | AGTGTTCGTCAAGAATGAGG | CCTCGCAGACGGAAAAC | cypCar_00039111 |
| Adaptive immune genes | |||
| igm | CACAAGGCGGGAAATGAAGA | GGAGGCACTATATCAACAGCA | AB004105 |
| igt1 | AAAGTGAAGGATGAAAGTGT | TGGTAACAGTGGGCTTATT | AB598367 |
| igt2 | GATTCTACTGGGT8CTTCAC | GACATCACTCAACTC8TTCT | AB598368 |
| zap70 | GGAACAAGCCATCATTAGCC | GTCGTCTCTCACCCTCCTG | Scaf 2523 and 63374 |