FIGURE 4.

Streptococcus mutans growth inhibition from LBO bioreactor fraction. Tendency curve of S. mutans inhibition by the oil sampled from the 50% LBO/broth (V2/V1) biphasic cultures of S. pyogenes and S. salivarius K12, in the bioreactor system at onset (T0) and after 8 h (T8) incubation. The experiment was performed at 37°C according to the Kirby-Bauer Agar diffusion test using 0.05 mL of oil. Data were collected after 24 h incubation. The third level polynomial curve obtained with three different measures showed a regression coefficient R² = 1.