Figure 1.

Conditional loss of Arid3a and B cell development. (A) Relative expression of Arid3a in B cell subsets in the bone marrow (common lymphoid progenitor and fractions A to E), spleen (transitional and mature stages), and peritoneal cavity using data from the ImmGen database. (B,C) Flow cytometry of bone marrow B cell development in Arid3a^fl/fl, Arid3a^fl/+ Cd79a^Cre/+, and Arid3a^fl/fl Cd79a^Cre/+ mice. Flow cytometry plots are shown on the left. Absolute cell numbers for B1 (CD19⁺B220^lo), B2 (CD19⁺B220⁺), immature B (CD19⁺B220⁺IgM⁺IgD⁻), recirculating B (CD19⁺B220⁺IgM⁻IgD⁺), pro-B (CD19⁺B220⁺IgM⁻IgD⁻ c-Kit⁺CD25⁻), and pre-B (CD19⁺B220⁺IgM⁻IgD⁻c-Kit⁻CD25⁺) cell populations are shown on the right. Error bars represent SEM and n = 17–24 for each group. p-Values were determined by Student’s t-test and fold changes are indicated. (D) Deletion of the conditional Arid3a allele determined by RT-polymerase chain reaction in sorted pro-B cells from the bone marrow of Arid3a^fl/+ Cd79a^Cre/+ and Arid3a^fl/fl Cd79a^Cre/+ aged 4–6 weeks of age. The full length wild-type and floxed allele (WT/Fl) and exon 4 deleted allele (ΔE4) are indicated. HPRT was used as a loading control.