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Doubled haploid (DH) technology. (A), Comparison between conventional breeding and DH technology; (B), Diagram of three major DH technologies adopted in crop breeding: anther culture, microspore culture and chromosome elimination. CD, Chromosome doubling with chemical treatment; (C), Anther culture in wheat. a, Anthers on a flower; b, Anthers cultured on medium; c, Callus formation; d, Initiation of differentiation; e, Differentiation of roots and shoots.
