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(A) Hepatic macrophages were identified by F4/80 immunohistochemistry. Mice with established fibrosis treated with CM, EVDM, and EV had significantly lower percentage of F4/80 macrophages in the liver compare to CCl4 only. Scale bar = 200 μm, 10× magnification. (B) Quantification of liver macrophage density using ImageJ software. The data are represented as mean ± SEM. n = 6–8 per group, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
