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. 2017 Oct 24;8:748. doi: 10.3389/fphar.2017.00748

FIGURE 7.

FIGURE 7

(A) Representative FACS plot of macrophages at 24-h post treatment. Cells were pre-gated based on size and viability. Quantification of the percentage of total number of cells gated from the FACS plot of (B) untreated M1-differentiated (LPS/INF-γ) macrophages or treated with hAEC-CM, EVDM, and EV, (C) untreated macrophages or treated with hAEC-CM, EVDM, and EV and (D) untreated M2-differentiated macrophages (IL-4/IL-13) or treated with hAEC-CM, EVDM, and EV. M1 and M2 differentiations were determined by CD86 and CD206, respectively. The data are represented as mean ± SEM. Each analysis was based on biological replicates of hAEC-EV, CM, and EVDM obtained from different hAEC, n = 3; P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.