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Controls for immunolabeling with tyramine antiserum. We used three sections of the subesophageal ganglion (SEG) that contain ventral median group cells (VUM). The sections were labeled with one of the following: (A) tyramine antiserum; (B) tyramine antiserum pre-incubated with conjugated tyramine-bovine serum albumin (BSA); (C) tyramine antiserum pre-incubated with octopamine-BSA. Scale bar: 25 μm.
