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. 2017 Oct 24;11:77. doi: 10.3389/fnsys.2017.00077

Figure 5.

Figure 5

Anti-AmTyr1 labeled synapses of the olfactory receptor neuron (ORN) axons in the antennal lobe glomerulus. (A,B) Triple immunofluorescence labeled with anti-AmTyr1 antibodies (magenta), neurobiotin tracer in ORNs (green) and anti-synapsin (blue). (B) Images are higher magnifications of details from corresponding squares indicated in (A). (A1,B1) Anti-AmTyr1 immunostaining expressed in the cortex of glomeruli (magenta). (A2,B2) The ending of the (ORNs, green) revealed by neurobiotin injections into antenna. Anti-AmTyr1 in glomeruli (A1,A3,A5,B1,B3,B5, magenta) is in ORN endings (A2,A3,A5,B2,B3,B5, green) co-labeled with anti-synapsin (blue, A4,A5,B4,B5). The white color in merged images (A3,A5,B3,B5) revealed anti-AmTyr1 co-stained in the ORN together with synapsin. The white arrows in (B1–B5) indicate co-localization with ORN endings by both anti-AmTyr1 and anti-synapsin; yellow arrow shows co-localization anti-AmTyr1 with synapsin but not with neurobiotin. Scale bar: A = 10 μm; B = 2 μm.