Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 24;11:77. doi: 10.3389/fnsys.2017.00077

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Sinakevitch, Daskalova and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Anti-AmTyr1 immunostaining in the antennal nerve. (A) General view of the antennal segments 5 and 6, neurobiotin was injected in the antennal lobe, and the image was obtained by overexposure with the confocal gain to illustrate different types of sensilla (tC–tricoid sensilla type C; Arrow indicates sensilla placodea (p). (B) Details of the ventral area of the antenna at higher magnification (tA, tB1, tricoid sensilla type A and B1 respectively, b-basiconic sensilla). (C) The section via antenna illustrates merged images of the group of ORN cell bodies and various processes labeled with neurobiotin tracer (green) anti-AmTyr1 (magenta), and 4′,6-diamidino-2-pheylindole (DAPI), marking the nucleus, P-indicate the fibers in the sensilla placodea. (D) Images present higher magnifications of details from corresonding squares indicated in (C). (D1) shows of cell bodies and processes labeled with neurobiotin (green) and anti-AmTyr1 staining (magenta, single staining) and nuclei (blue, DAPI). (D2) illustrates only neurobiotin labeled processes (green) and nuclei (DAPI, blue). (D3) illustrates only anti-AmTyr1 (magenta) and nuclei (DAPI). (D4) shows the nuclei staining. The arrow indicates cell bodies that have co-staining with AmTyr1 and neurobiotin. (E) Anti-AmTyr1 (E1 single image, magenta) is in the area of sensilla placodea (p) with dendrites of ORNs labeled with neurobiotin (green E2). The absence of the white staining in merged image (E3) demonstrates that AmTyr1 does not co-label dendrites of labeled ORNs. Scale bar: A = 100 μm; B,E = 20 μm; C = 25 μm; D = 10 μm.