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. 2017 Aug 20;30(4):351–358. doi: 10.1293/tox.2017-0031

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©2017 The Japanese Society of Toxicologic Pathology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/）.

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Fig. 2. — Analysis of a cellular vacuole using Image-Pro Plus. (A) Analyzed image of the lipid-derived vacuole. Lipid droplets (green-colored objects) were recognized by the criteria of size and roundness, and the size was measured. Vessels, the bile duct, and sinusoidal spaces were excluded. Bar = 50 μm. (B) The total area of the lipid droplet per size in the hepatocyte with various grades of lipid deposition. (C) Representative images of tissue specimens with various grades (left, center, and right images: normal, −; very slight, ± ; and slight, +, respectively) of lipid deposition. Bar = 100 μm. (D) Quantification of the area of mucin in the intestinal mucosa. Left image: original image. Right image: analyzed image of mucin in the intestinal mucosa. The outline of the intestinal mucosa was drawn manually using the AOI setting (green line) in order to exclude nonspecific objects in the outside region, and then the inside region was analyzed. The mucin (light blue-colored objects) was recognized by the criteria of size. Bar = 200 μm.