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. 2017 Oct 25;8:2094. doi: 10.3389/fmicb.2017.02094

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Copyright © 2017 Zhao, Feng, Lü, McNally and Zong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The circular intermediate formed by ISApl1. Genetic context of mcr-1 on the IncP plasmid pMCR_1511 is shown. Orfs that encode hypothetical proteins with unknown function are indicated in white except that the one disrupted by the ISApl1-formed composite transposon on pMCR_1511 is shown in black (Δ1 and Δ2). The 2-bp direct repeat (AC) abutting the ISApl1-formed composite transposon on pMCR_1511 is shown. On pMCR_1511, the ISApl1 downstream of mcr-1 was interrupted by Tn3, which was interrupted by IS26.