Figure 2.

Inhibition of ciclooxigenase-2 impairs T. cruzi entry into DC-PBMC Percentage of infected cells from the interaction process between DC-PBMC treated for 1 hour with ASA (A) or CEL (B) [0.312, 0.625, and 1.25 μM] and exposed to 5:1 trypomastigotes of T. cruzi (Y strain) during 18 h. After DC-PBMC were fixed with methanol and stained with Giemsa stain. Quantification was carried out under a light microscope where the number of intracellular parasites was counted in a total of least 200 cells. MTT assay to measure cell viability in DC-PBMC after treatment with ASA (C) or CEL (D). H₂O₂ (1,000 μM) was used as positive control. Values are the mean ± standard error of two experiments. DMSO, Dimethyl sulfoxide 0.05%. Results are the mean ± standard error for duplicate determinations, with six blood donors in each experiment. Means not sharing letter are significantly different (P < 0.05, one-way ANOVA with Tukey's post-test).