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. 2017 Oct 25;8:2020. doi: 10.3389/fmicb.2017.02020

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Copyright © 2017 Lonien, Malvezi, Suzukawa, Yamauchi, Yamada-Ogatta, Rizzo, Bordignon and Pinge-Filho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PGE₂ restored the invasiveness of T. cruzi in DC-PBMC previously treated with CEL. DC-PBMC were treated for 30 min with separately either with or without PGE₂ (1 or 10 μM) alone or in combination with ASA (A) or with CEL (B) and exposed to 5:1 trypomastigotes of T. cruzi (Y strain) during 18 h. After cells were fixed with methanol and stained with Giemsa stain. Results are the mean ± standard error for duplicate determinations, with three blood donors in each experiment. Means not sharing letter are significantly different (P < 0.05, one-way ANOVA with Tukey's post-test).