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. 2017 Oct 25;8:2020. doi: 10.3389/fmicb.2017.02020

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Copyright © 2017 Lonien, Malvezi, Suzukawa, Yamauchi, Yamada-Ogatta, Rizzo, Bordignon and Pinge-Filho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nitric oxide (NO) production by DC-PBMC infected with T. cruzi. DC-PBMC were treated for 1 h with ASA (A) or CEL (B). After treatment, the cells were washed and incubated with 5:1 trypomastigotes for 24 h. Nitrite levels in the supernatant were measured by Griess reaction. Results are the mean ± standard error of duplicate determinations. Three independent experiments were performed, with six individuals in each experiment. Means not sharing letter are significantly different (P < 0.05, one-way ANOVA with Tukey's post-test).