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. 2017 Oct 25;8:1351. doi: 10.3389/fimmu.2017.01351

Figure 1.

Figure 1

In response to human rhinoviruses strain 16 (RV16) stimulation, plasmacytoid dendritic cell (pDC)-depleted peripheral blood mononuclear cells (PBMCs) produce significantly less interferon (IFN)-α than intact PBMC. PBMC were either depleted of pDC using CD303 immunomagnetic beads or left intact. Efficiency of pDC depletion was examined with flow cytometry (A). PBMC (5 × 105 cells/well) were then cultured in the presence/absence of RV16 (MOI = 1) for 24 h at 37°C. IFN-α production in culture supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Data shown are for the net RV16 simulated IFN-α concentration following subtraction of the IFN-α concentration in the absence of RV16 (media control). The dotted lines represent the median of each group (n = 15). IFN-α concentrations in pDC-depleted PBMC were significantly lower than intact PBMC (p < 0.001) (B).