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. 2017 Oct 25;8:1351. doi: 10.3389/fimmu.2017.01351

Figure 5.

Figure 5

Validations experiments using real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cell (PBMC) and plasmacytoid dendritic cell (pDC)-depleted PBMC (n = 15) were cultured for 24 h in the absence or presence of human rhinoviruses strain 16 (RV16). mRNA expression of IRF7, IL-27p28, EBI3, IL-12p35, IL-15RA, IFI27, and CD303 was determined by RT-PCR. Data represent fold change relative to median of controls against the average of two reference genes (UBE2D2 and B2M) (A). IL-6 and interferon (IFN)-γ were measured in culture supernatant by ELISA. Net cytokine values are shown (RV16-stimulated cultures minus the unstimulated cultures) (B). All data represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (I, intact PBMC; P, pDC-depleted PBMC).