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. 2017 Oct 25;8:2081. doi: 10.3389/fmicb.2017.02081

Figure 6.

Figure 6

The cytotoxicity and cytokine mRNA expression in the C. perfringens-infected cells pretreated with lactobacilli. Cells were pre-incubated with L. acidophilus or L. fermentum (MOI = 1) for 2 h and then stimulated with C. perfringens (MOI = 0.1) for 6 h. Following infection, LDH released from cells to culture medium were detected (A). The mRNA expression of IL-6 (B), IL-8 (C), iNOS (D), LITAF (E) and IL-1β (F) were determined by qRT-PCR analysis. Data are presented as mean ± standard error (n = 3). **P < 0.01. La, L. acidophilus; Lf, L. fermentum; Cp, C. perfringens; IL, interleukin; iNOS, inducible nitric oxide synthase; LITAF, lipopolysaccharide induced TNF factor.