Fig. 1. VPA induced the expression of TERT mRNA and protein in cultured NPCs. (A) The expression of TERT protein was examined by Western blot, and the expression of Tert mRNA was examined by RT-PCRin VPA-treated NPCs. Results are mean±S.E.M. (n=3). NPCs were cultured with embryos fromthree different pregnant rats. ^*p<0.05, ^**p<0.01, ^***p<0.001 vs. control. (B) Immunocytochemistry images of VPA-treated NPCs to detect Pax6 and TERT expressions. NPCs were cultured with embryos from three different pregnant rats. Scale bar is 100 µm.