Fig. 2. Prenatal VPA exposure induced TERT expression in the mPFC region. (A) The expression of TERT protein was examined by Western blot, and the expression of Tert mRNA was examined by RT-PCRin embryos exposed to VPA-treatment in pregnant mothers. Results are mean±S.E.M. (n=3). Four rat embryos were randomly chosen from three different pregnant rats. ^*p<0.05, ^**p<0.01 vs. control. (B) Immunocytochemistry images of VPA-exposed embryonic brains to detect nestin and TERT expressions. One embryo was randomly chosen from three different litters. Scale bar; 100 µm.