Fig. 5. Expression of excitatory/inhibitory synaptic proteins after Tert siRNA transfection in cultured NPCs. (A) The expression of sequential protein markers known for induction of glutamatergic differentiation was analyzed 1 and 7 days after transfection of Tert siRNA. (B) Twelve days later, the expressions of pre- and post-synaptic markers were analyzed. Results are mean±S.E.M. (n=3). NPCs were cultured with embryos from three different pregnant rats. ^**p<0.01, ^***p<0.001 for control group vs. VPA-treated group; ^##p<0.01, ^###p<0.001 for control siRNA group vs. Tert siRNA group; ^α'p<0.01, ^αp<0.001 for Tert siRNA control group vs. Tert siRNA VPA-treated group, as determined by Bonferroni's posthoc comparisons test.