Fig. 7. Expression of transcription factors regulating neuronal subtype differentiation after modifying Tert expression. (A,B) In utero transfection was used for knock-down of Tert in the embryonic mice brain. The fluorescent-labeled Tert siRNA was delivered into subventricular zone of E14.5 mice brain, and electroporated mice brain was isolated at E18.5 for immunohistochemistry. Ngn2 is a transcription factor for regulating glutamatergic differentiation, and Calbindin D is a marker for processing GABAergic differentiation in neurons. (C) Brain expression of nestin and Pax6 from WT and TERT-tg mice at E12.5 (Scale bar, 500 µm).