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. 2017 Oct 25;8:1824. doi: 10.3389/fpls.2017.01824

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Copyright © 2017 Ning, Wu, Zhang, Wu, Niu, Yang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2-DE profiles, abundance, and comparison of SSPs in maize embryos of hybrids and their parents. Maize embryo SSPs (600 μg) were resolved by IEF using 11 cm pH 4–7 IPG dry Strip. Secondary SDS-PAGE was carried out on a 12.5% resolving gel. (A) Spots of relatively abundant protein in hybrids and their parents with at least two-folds change in abundance are indicated with red arrows. Blue boxes indicate the regions concentrated with differential SSPs. (B,C) Indicating log2 ratios of Zheng 58 × Chang 7-2 and Chang 7-2 × Zheng 58 to their parents blue represents Zheng 58 and red represents Chang 7-2. Log2 ratio > 1 represents more than two-folds of abundance change. Spot volumes were determined using the PDQuest software.