Table 2.
Identification of relatively abundant SSPs in maize embryos by MS/MS among hybrids and parental inbred lines.
| Spot | Protein | NCBI accession | UniProtKB accession | Score | Coverage (%) | Matched peptide sequences |
|---|---|---|---|---|---|---|
| 1 | Globulin-1-like seed storage protein, partial | AQL09051 | A0A1D6PIB9 | 833 | 75.2 | VGGQVVEK; TPPPIIAYNPEEEKGDK; VTSIEEESSEQSSLEVER; IYAIFTSEGINADDPSKPK; GKVTSIEEESSEQSSLEVER; VVAESEAGSVSAVDVADAAGTAYR; LRIYAIFTSEGINADDPSKPK; RVVAESEAGSVSAVDVADAAGTAYR; GDVYNFEQGSILYIQSYPNASR; GFETDVLR; VEAYSSVSNLVK; LGFGVKPEVVEAIK; LHFITMDPGALFLPVQLHADMVFYVHSGR |
| 2 | Globulin-1-like seed storage protein, partial | AQL09051 | A0A1D6PIB9 | 768 | 71.8 | LGFGVKPEVVEAIK; TPPPIIAYNPEEEK; TPPPIIAYNPEEEKGDK; VTSIEEESSEQSSLEVER; IYAIFTSEGINADDPSKPK; GKVTSIEEESSEQSSLEVER; VVAESEAGSVSAVDVADAAGTAYR; LRIYAIFTSEGINADDPSKPK; RVVAESEAGSVSAVDVADAAGTAYR; GDVYNFEQGSILYIQSYPNASR; GFETDVLR; VEAYSSVSNLVK; LHFITMDPGALFLPVQLHADMVFYVHSGR |
| 3 | Globulin 2 precursor | NP_001295419 | Q7M1Z8 | 519 | 37.1 | VVMLLSPVVSTSGR; FTHELLEDAVGNYR; GQGYFEMACPHVSGGR; AFLQPSHYDADEVMFVK; LLAFGADEEQQVDRVIGAQK; EGDVMVIPAGAVVYSANTHQSEWFR; REEWEK; VAELEAAPR; QSKGEITTASEEQIR; GEITTASEEQIR; FEEFFPIGGESPESFLSVFSDDVIQASFNTR |
| 4 | Globulin-1 S allele precursor | ACG48473 | B6UGJ0 | 448 | 48.1 | REEWEK; EGEGVIVLLR; GEITTASEEQIR; GSRGEGGDSGSSSSSK; VVMLLSPVVSTSGR; FTHELLEDAVGNYR; GQGYFEMACPHVSGGR; TFLQPSHYDADEVMFVK; VAELEAAPR; VLERFTHELLEDAVGNYR; LLAFGADEEQQVDRVIGAQK; EGDVMVIPAGAVVYSANTHQSEWFR; KPTHSNSHGRHYEITGDECPHLR; FEEFFPIGGESPESFLSVFSDDVIQASFNTR |
| 5 | Globulin-1 S allele precursor | ACG48473 | B6UGJ0 | 450 | 42.8 | TFLQPSHYDADEVMFVK; QSKGEITTASEEQIR; VLERFTHELLEDAVGNYR; FTHELLEDAVGNYR; LLAFGADEEQQVDRVIGAQK; VVMLLSPVVSTSGR; FEEFFPIGGESPESFLSVFSDDVIQASFNTR; EGEGVIVLLR; GEITTASEEQIR; REEWEK; VAELEAAPR; EGDVMVIPAGAVVYSANTHQSEWFR; GQGYFEMACPHVSGGR |
| 6 | Globulin-1 S allele precursor | ACG48473 | B6UGJ0 | 449 | 42.8 | GQGYFEMACPHVSGGR; TFLQPSHYDADEVMFVK; FTHELLEDAVGNYR; EGEGVIVLLR; LLAFGADEEQQVDRVIGAQK; EGDVMVIPAGAVVYSANTHQSEWFR; VFEKQSK; REEWEK; VAELEAAPR; QSKGEITTASEEQIR; GEITTASEEQIR; VVMLLSPVVSTSGR; FEEFFPIGGESPESFLSVFSDDVIQASFNTR; |
| 7 | 16.9 kDa class I heat shock protein 1 | ACG36285 | B6TGQ2 | 275 | 59.9 | ADLPGVKK; SSGQFVRR; FRLPENAK; AALENGVLTVTVPKAEVK; AALENGVLTVTVPK; VEVEDGNVLLISGQR; EEVKVEVEDGNVLLISGQR; SSVFDPFSVDLFDPFDSMFR; ETPEAHVFK; RSSVFDPFSVDLFDPFDSMFR |
| 8 | 16.9 kDa class I heat shock protein 1 | ACG31332 | B6T2J9 | 263 | 70.1 | AALENGVLTVTVPK; VEVEDGNVLLISGQR; SIVPPSLSSSAASETAAFASAR; ETPEAHVFK; EEVKVEVEDGNVLLISGQR; ADLPGVKK; SSGQFVRR; FRLPENAK; SSVFDPFSVDLFDPFDSMFR |
| 9 | Globulin-2 | ONM10183 | A0A1D6L7G1 | 441 | 37.0 | GHGREEEEER; VFLAGTNSALQK; LLAFGADEEQQVDR; EEEEEREEEQGGGGGQK; VFLAGTNSALQKMDRPAK; EGSVIVIPAGHPTALVAGEDK; IREGSVIVIPAGHPTALVAGEDK |
| 10 | Globulin-2 | ONM10183 | A0A1D6L7G1 | 467 | 36.1 | NLAVLCFEVNASFDDK; EEEEEREEEQGGGGGQK; EGSVIVIPAGHPTALVAGEDK; LLAFGADEEQQVDR; GHGREEEEER; VFLAGTNSALQK; IREGSVIVIPAGHPTALVAGEDK |
| 11 | Globulin-2 | ONM10183 | A0A1D6L7G1 | 205 | 31.1 | EEEQGGGGGQK; MFVKEGEGVIVLLR; EGSVIVIPAGHPTALVAGEDK; VFLAGTNSALQK; LLAFGADEEQQVDR; IREGSVIVIPAGHPTALVAGEDK |
| 12 | Globulin-2 | ONM10183 | A0A1D6L7G1 | 371 | 49.2 | MFVKEGEGVIVLLR; LLAFGADEEQQVDR; HYEITGDECPHLR; EEEEEREEEQGGGGGQK; EGSVIVIPAGHPTALVAGEDK; GSMMAPSYNTR; VFLAGTNSALQK; LLDMDVGLANIAR; IREGSVIVIPAGHPTALVAGEDK |
The parameters of Mascot search were as follows: Type of search: combined (MS + MS/MS); Enzyme: trypsin; Fixed modifications: carbamidomethyl (C); Dynamical modifications: oxidation (M); Mass values: monoisotopic; protein mass: unrestricted; Peptide mass tolerance: ±100 ppm; Fragment mass tolerance: ±0.4 Da; Peptide charge state: 1+; Max missed cleavages: 1.