Figure 5.

Troxerutin increases kidney Nrf2 activity in BDE-47-treated mice. (a) The activities of Keap1, cytoplasmic Nrf2, and nuclear Nrf2 were detected by Western blot assay. (b) Nrf2 ubiquitylation was evaluated by Western blot analysis after Nrf2 immunoprecipitation. (c) Nrf2 immunohistochemical staining. Green fluorescence represents positive signal, and DAPI was used to stain the nuclei blue. Scale bar, 50 μm. White arrow indicates positive signal which was placed in the upper right corner. Data were expressed as mean ± SEM (n = 5). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 versus BDE-47-treated group. ^##p < 0.01 versus control group.