Figure 9.

P2X₇R expression in M1 and M2 macrophages. Representative double‐immunostained images of CD68 (A), CD86 (B) and CD163 (C) (red); P2X₇R (green); and DAPI (blue) for nuclei (magnification ×400). Bar = 30 μm. (D) Fraction of P2X₇R‐positive M0, M1 and M2 macrophages. Representative protein expression levels of P2X₇R (80 kD) in M1 (E and G) and M2 (F and H) macrophages as determined by Western blotting. Protein levels were quantified relative to GAPDH (37 kD) levels. The data are expressed as the mean ± S.D. of two experiments. *P < 0.05 and **P < 0.01 versus the M0 (D) or sham groups (G and H).