Figure 6.

LAP-dependent BMDC cytokine response to commensal yeasts. (A) WT, E226, and E230 BMDCs were incubated with the indicated yeasts at a MOI of 5. After 16 h incubation, the supernatant was harvested and the amount of TNFα and IL-1β was measured by ELISA. (B) WT, E226, and E230 BMDCs were stained with anti-Dectin-1 and 2 Abs (red) or the isotype control Abs (blue). The stained cells were analyzed by flow cytometry. Data shown are the mean of triplicates ± SD from one representative experiment and reproducible in three independent experiments. Statistical analyses were performed by one-way ANOVA followed by Tukey’s test.