Figure 5.

BCAA regulates KLF15 expression at transcriptional level. (A) Quantitative PCR was performed to measure the mRNA level of KLF15 in cells pretreated with actinomycin D (5 μg/ml) or DMSO for 30 min before BCAA starvation (−) for 6 h. (B) Luciferase assay results from MEF cells transfected with luciferase reporter vectors with (+) or without (−) KLF15 3'UTR with (+) or without BCAA (−). (C) Quantitative PCR was performed to measure the relative mRNA level of KLF15 in cells treated with actinomycin D (5 μg/ml) for indicated time in the presence (con) or absence of BCAA (BCAA starvation). Data are means ± SE for three individual samples. ^**p < 0.01.